Naslov
Effect of diets enriched with pumpkin-seed, olive and fish oil or lard on mice liver regeneration

Autori
Čanadi Jurešić, Gordana ; Broznić, Dalibor ; Marinić, Jelena ; Tota, Marin ; Čedomila, Milin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Helga Medić - : Hrvatsko društvo prehrambenih tehnologa, biotehnologa i nutricionista, 2011, 194-194

ISBN
978-953-99725-3-8

Skup
7th International Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Opatija, Hrvatska, 20.09.2011. - 23.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
liver regeneration; diets; fat

Sažetak
Introduction: The liver is remarkable organ, given its inherent capacity to fully restore itself after significant hepatic tissue loss either from partial hepatectomy (pHx) or acute liver injury. The best experimental model for the study of liver regeneration is a pHx, in which part of the liver is surgically removed. Fast liver growth, following pHx is well defined process, which involves the concerted action of extra and intracellular factors resulting in cell replication and its inhibition at the time when the entire liver mass is restored. Nutritional factors are one of the factors involved in this process. The aim of the study was to compare the effect of diets enriched with different oils or lard on liver regeneration, following 1/3 pHx in mice. Methodology: Male C57/Bl6 mice, aged 8-10 weeks, were fed by diets enriched either with pumpkin-seed, olive or fish oil or lard (5% addition to standard pellet, w/w) during the 3 weeks and afterwards subjected to 1/3 pHx. 2, 4, 24, 48 and 168 hours later mice were sacrificed and the remaining liver analysed. Total liver lipids were extracted and then separated into fractions by solid-phase extraction. Results and Discussion: Each of the experimental diets affected liver regeneration differently. In all diets, except in lard enriched diet, liver had regained much of its lost mass 48 hours after pHx, and regeneration finished after 196 hours. In all diets, except in fish oil enriched diets, regenerating liver accumulated extra fat (increment of neutral lipid fraction) in first few days after pHx, with the highest value at 24 hours after pHx. Fish oil enriched diet provoked the fastest liver regeneration (after 48 hours >90% of the original mass) and simultaneously, reflected itself in the lowest total liver lipid content. Conclusions: Enrichment of diets with fats, particularly fish oil, can improve liver regeneration.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA