Naslov
Detection of human papillomavirus (HPV) type 6, 16 and 18 in head and neck squamous cell carcinomas by in situ hybridization

Autori
Cerovac, Željka ; Šarčević, Božena ; Kralj, Zvonimir ; Ban, Jasna

Izvornik
Neoplasma (0028-2685) 43 (1996), 3; 185-194

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HPV ; head and neck carcinoma ; histological grade ; in situ hybridization

Sažetak
77 squamous cell carcinomas (10 oral cavity, 15 tongue, 26 pharynx with tonsil, and 26 larynx) with different grading were analyzed for the presence of HPV DNA by in situ hybridization. Positive signals were found on the nuclei of cancer cells in 25 (32.5%), in the epithelia adjacent to squamous cell carcinomas in 2 (8.7%), and in the resected margins in 1 (4.3%) case. HPV DNA positive signals were obtained in 42% of laryngeal, 34% of pharyngeal, in 20% of oral, and 20% of tongue carcinomas. Out of 25 HPV positive carcinomas a single HPV type was detected in at least 11 (44%), and double or multiple infection in 9 (36%) cases ; altogether, HPV DNA 6 was determined in 15 (60%), and HPV DNA 16 and/or 18 in 17 (68%) head and neck tumors. The detection rate of HPV 6 was lower than of HPV 16 and/or 18 for tumors in oral cavity, tongue and larynx. Out of 25 HPV DNA positive carcinomas 21% were graded as G1, 27% as G2, and 44% were G3. The results indicate that HPV may be involved in the pathogenesis of head and neck squamous cell carcinomas.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA