Naslov
Optimization of laccase production by Trametes versicolor cultivated on industrial waste

Autori
Tišma, Marina ; Žnidaršič-Plazl, Polona ; Vasić-Rački, Đurđa ; Zelić, Bruno

Izvornik
Applied biochemistry and biotechnology (0273-2289) 166 (2012), 1; 36-46

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
laccase production; industrial waste; medium optimization; genetic algorithm; Trametes versicolor; microparticles

Sažetak
Laccases are very interesting biocatalysts for several industrial applications. Their production by different white rot fungi can be stimulated by a variety of inducing substrates and the use of lignocellulosic wastes or industrial by-products is one of the possible approaches to reduce production costs. In this work, various industrial wastes were tested for laccase production by Trametes versicolor MZKI G-99. Solid waste from chemo-mechanical treatment facility of a paper-manufacturing plant showed the highest potential for laccase production. Enzyme production during submerged cultivation of T. versicolor on the chosen industrial waste has been further improved by medium optimization using genetic algorithm. Concentrations of five components in the medium were optimized within sixty shake-flasks experiments, where the highest laccase activity of 2, 378 U dm-3 was achieved. Waste from paper industry containing microparticles of CaCO3 was found to stimulate the formation of freely dispersed mycelium and laccase production during submerged cultivation of T. versicolor. It was proved to be safe and inexpensive substrate for commercial production of laccase and might be more widely applicable for metabolite production by filamentous fungi.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA