Naslov
Variability of stolbur phytoplasma strains infecting Croatian grapevine by multilocus sequence typing

Autori
Šeruga Musić, Martina ; Pušić, Petra ; Fabre, Anne ; Škorić, Dijana ; Foissac, Xavier

Izvornik
Bulletin of insectology (1721-8861) 64 (2011); S39-S40

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
16SrXII-A; MLST; phylogenetic analysis; tufB; vmp1; secY

Sažetak
In order to investigate the genetic variability among selected grapevine strains belonging to 16SrXII-A subgroup (stolbur) as a case study, multilocus sequence typing (MLST) approach was used. Three non-ribosomal house-keeping genes were analyzed: tufB, secY and the recently characterized variable vmp1 gene, encoding a putative membrane protein of stolbur phytoplasma. Restriction analysis of tufB gene amplicons revealed the presence of both tuf-a (VK-I) and tuf-b (VK-II) types, with the latter being more prevalent among stolbur strains from Croatian grapevine analyzed in this study. In genotyping of vpm1 gene, five different RFLP profiles were obtained including a mixed pattern observed in one sample. The vmp1 diversity was therefore important among selected strains. Phylogenetic analyses of secY sequences were in accordance with the results of tufB and vmp1 typing ; however in some of the analyzed strains incongruence was observed. Nevertheless, the results presented have shown the presence of considerable genetic variability among relatively small number of 10 selected stolbur isolates from grapevine and proved the importance of MLST application in differentiation of closely related strains.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA