Naslov
The effect of three irrigation techniques on the reduction of intracanal Enterococcus faecalis ex vivo

Autori
Bago, Ivona ; Toljan, Ivana ; Baraba, Anja ; Miletić, Ivana ; Anić, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Endodontic Journal 44(12) / Dummer P.M.H. - , 2011

Skup
15. Biennial Congress of the European Society of Endodontology

Mjesto i datum
Rim, Italija, 14.09.2011. - 17.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Enterococcus faecalis; irrigation; sodium hypochlorite

Sažetak
Aim To compare intracanal bacterial reduction promoted ex vivo by three irrigation techniques with regard to the standardized volume of NaOCl and standardized irrigation time. Methodology Forty-eight single-rooted teeth with straight canals of approximately the same volume were instrumented with ProTaper istruments and sterilized in plasma. Forty-two root canals were innoculated with an Enterococcus faecalis suspension, and the remaining six canals were filled with sterile broth and served as negative controls. After 48 h of incubation, 36 root canals were randomly distributed into 3 groups of 12 roots each according to the irrigation technique used ; group 1, conventional syringe irrigation using needle ; group 2, automated-dynamic irrigation using RinsEndo ; group 3, passive ultrasonic irrigation (PUI) using Piezon Master 400. The remaining six root canals served as positive controls. Antimicrobial efficacy of the irrigation techniques was analysed in regard of standardized volumes of 3% NaOCl (20 mL) in the first protocol, and in regard of standardized irrigation time (45sec) in the second protocol. Samples taken immediately after irrigation were cultured and the colony-forming units (CFUs) were counted. Results When the volume of the irrigant was standardized, RinsEndo was more efficacious than PUI (p<0.01), however, there were no statistically significant differences between other techniques. When the irrigation time was standardized, there were no significant differences between any irrigation technique (p>0.05). The highest percentage of minimal counts of E. faecalis CFUs was noted in RinsEndo group in both protocols. Conclusions On the basis of this ex vivo investigation, RinsEndo was superior to PUI only when the volume of the irrigant was standardized. However, RinsEndo seemed to be more beneficial because it caused higher bacterial reduction in both protocols when using the least amount of irrigant, and provided longer contact time.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA