Naslov
Highly repeated non-coding DNA in Tribolium castaneum (Coleoptera) genome

Autori
Pavlek, Martina ; Plohl, Miroslav ; Meštrović Radan , Nevenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
18th International Chromosome Conference, Delegate book, Manchester, UK. / - Manchester, 2011, 1-1

Skup
18th International Chromosome Conference

Mjesto i datum
Manchester, Ujedinjeno Kraljevstvo, 29.08.2011. - 02.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
satelitna DNA ; Tribolium castaneum
(satellite DNA ; Tribolium castaneum)

Sažetak
Many reports support involvement of satellite DNAs in complex centromeric regions, but little is known about their general structural and functional features. Recently, Tribolium castaneum became the first coleopteran species with sequenced genome and emerged as a powerful insect model organism, especially in comparative analysis with Drosophila melanogaster. However, satellite repeats are underrepresented in assembled outputs of genome projects and high-resolution view on their composition and sequential organization remains obscure. Results based on our in silico and experimental analysis of four different satellite DNAs, extracted from T. castaneum genome database, will be presented. Analysis of structure, organization, localization, as well as a comparative studies of these satellite families show monomer length between 166 and 370 pb, abundance from 0, 1 to 17 % and different sequence homogeneity of families. In addition, satellite DNAs exhibit different organizational forms, as randomly mixed monomer variants, higher-order-repeats (HOR) or as specific subfamily arrays. FISH experiments revealed either peri(centromeric) localization or dispersed distribution. We assume that understanding different features of these sequences in the Tribolium castaneum genome will help to gain a more comprehensive view of their possible role(s) in genome architecture and function.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA