Naslov
Usporedna citogenetička istraživanja vrsta Picea abies (L.) Karsten i Picea omorika (Pančić) Purk.
(Cytogenetic comparison of Picea abies (L.) Karsten and Picea omorika (Pančić) Purk.)

Autori
Siljak-Yakovlev, Sonja ; Cerbach, Malika ; Caulaud Joelle ; Papeš, Dražena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka priopćenja šestog kongresa biologa Hrvatske / Huber, Đ. - Zagreb : Hrvatsko biološko društvo, 1997, 87-88

Skup
Šesti Kongres Biologa Hrvatske

Mjesto i datum
Opatija, Hrvatska, 22.09.1997. - 26.09.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Picea abies; Picea omorika; oprugavanje kromosoma; FISH
(Picea abies; Picea omorika; chromosome banding; FISH)

Sažetak
A wild distributed species Picea abies and a paleoendemic (a tertiary relict) narrow localized Picea omorika have been cytogenetically investigated. Both of them showed identical chromosome number 2n=24, and morphologically similar karyotypes. We used some molecular cytogenetic techniques to find out whether any interspecific difference at chromosomal level exists. Two differential fluorescent banding techniques using A-T specific fluorescent dye Hoechst 33258 and G-C specific fluorescent dye Chromomycin A3, were applied as well as a fluorescent in situ hybridization method for detection of 18S and 5S rDNA gene clasters. The nuclear DNA content and the amount and relation between A-T and G-C base pairs were determined by flow cytometry technique. Obtained results showed a significant differentiation on chromosomal and genomic level which could explain the reproductive barriers between investigated Picea species.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA