Naslov
Optimization of betanin yield in tumor cell line of sugar beet Beta vulgaris L.

Autori
Križnik, Bojana ; Pavoković, Dubravko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th Symposium of Biology Students in Europe (SymBioSE), SymBioSE abstract booklet / - Basel, 2011, 33-34

Skup
15th Symposium of Biology Students in Europe (SymBioSE)

Mjesto i datum
Basel, Švicarska, Switzerland

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sugar beet; betanin; beta vulgaris; yield

Sažetak
Industrial crops, some of which are also used as food, are subjected to environmental changes that influence their yield. To overcome the problems of fluctuating yield and world problem of food deficit, there have been many attempts to make in vitro systems for industrial purposes. One example of such system is tumor cell line of sugar beet that is genetically transformed using bacteria in order to improve production of a pigment used in food industry, betanin. Betalains are plant pigments found in certain members of plant family Caryophylalles and some higher fungi. Betanin belong to a pigment group of red-violet betacyans and is used as a natural dye in food industry. Main source of betanin is red beet (Beta vulgaris L.), whose yield largely depends on environmental conditions. The aim of this work is to initiate betanin biosynthesis in sugar beet cells, which could be used as an alternative source of betanin for purposes of food industry. Sugar beet tumor cell lines, that have a T-DNA of bacteria Agrobacterium tumefaciens wild strain B6S3 inserted in genome, produce betanin. Effect of medium composition (LS and PG), sucrose concentration, and elicitors calcium ions and yeast extracts on betanin production and betanin yield was investigated. Results showed that increased concentration of sucrose increases betanin yield up to 30%, whereas betanin yield is 10% higher on LS medium than on PG medium. Increased concentration of calcium ions added in early exponential phase of cell growth slightly increased pigment production. Yeast extract at 0, 25% concentration did not increase betanin production, 1, 25% yeast extract caused excretion of betanin at day 4 and cell necrosis at day 7. Higher activity of a key enzyme in betanin biosynthesis, tyrosinase, was detected in betanin producing tissues, and a relation between enzyme quantity in tissue and betanin yield was observed. In conclusion, we obtained a stable culture that produces betanin in a short time, which could be a promising alternative production system.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA