Naslov
Genomic in situ hybridization in viviparous onions (Allium cepa var. viviparum)

Autori
Papeš, Dražena ; Puizina, Jasna ; Klein, Franz ; Schweizer, Dieter ; Bohanec, Borut ; Javornik, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Plant and Animal Genome V / Heller S.R. - New York (NY) : Scherago International, Inc., 1997

Skup
The International Conference on the Status of Plant & Animal Genome Research

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 12.01.1997. - 16.01.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Allium cepa var. viviparum; genomic fluorescent in situ hybridization; genome organization

Sažetak
Genomic fluorescent in situ hybridization was applied in the study of the genome organization and evolution of diploid (2n=2x=16) and triploid (2n=3x=24) viviparous onions (Allium cepa var. viviparum), traditionally cultivated in the seaside regions of Croatia. Genomic DNAs from three diploid Allium species: A. cepa L. (2n=16), A. fistulosum L. (2n=16), A. roylei Stearn (2n=16) and triploid Indian Allium "Pran" were used as probes in this study. In A. cepa var. viviparum, 2x, biotinylated DNAs from A. cepa and A. fistulosum labelled eight longer and eight shorter chromosomes respectivelly, thus confirming hybrid constitution of the diploid viviparous onion karyotype. In A. cepa var. viviparum, 3x ("Ljutika"), DNA from A. cepa labelled only 8 longer chromosomes, whereas DNA from A. roylei labelled entire length of 8 rather short chromosomes and the telomeres of a few other chromosomes. A. fistulosum failed to label triploid karyotype. These results imply that besides two identified genomes (A. cepa and A. roylei) in the triploid karyotype the third genome, originating from a still unknown Allium species, must have been involved. Identification of the genome of A. roylei, endemic Himalayan Allium species, in triploid karyotype of "Ljutika" indicates that this natural triploid onion form was introduced in Croatia and Europe from the Far East.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA