Naslov
Genetic variation and virulence of Cryphonectria hypovirus (CHV-1) from Croatia

Autori
Krstin, Ljiljana ; Rigling, Daniel ; Ježić, Marin ; Ćurković-Perica, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS 2011, abstract book / Schrenzel, J. ; Zbinden, R. ; Schink, B. - Ženeva : Kenes Group, 2011, 115-115

Skup
4TH Congress of European Microbiologists

Mjesto i datum
Ženeva, Švicarska, 26.06.2011. - 30.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biological control; chestnut blight; Cryphonectria parasitica; fungal virus; hypovirulence

Sažetak
Chryphonectria hypovirus 1 (CHV-1) is a successful biological control agent for the chestnut blight fungus Cryphonectria parasitica, which has been introduced into Croatia in 1950s. In order to investigate the incidence and the genetic variation of CHV-1, we sampled 338 isolates of C. parasitica from 10 Croatian chestnut populations. Hypovirus-infected isolates of the fungus were found in all populations with an overall incidence of 43.5%. The highest incidence of virus-infected isolates were observed in continental part of Croatia (66.6%) and the lowest in the coastal part of the country (12.7%). Thirty-six hypoviral isolates were characterized by RT-PCR/RFLP analysis and sequencing of hypoviral open reading frame (ORF-A). Both analyses revealed that all hypoviral isolates belonged to the Italian subtype of CHV-1. They were closely related to each other and to isolates from other European countries as well as to isolates sampled in Croatia 25 years ago. Effect of different hypovirus isolates on the host was assessed by measuring growth and sporulation of infected C. parasitica strains in vitro. Only one hypoviral isolate sampled on island Cres reduced fungal growth and sporulation significantly and is therefore considered as a potential agent for human-mediated biological control in chestnut orchards.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA