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Pregled bibliografske jedinice broj: 517162

Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins


Strelec, Ivica; Has-Schön, Elizabeta; Vitale, Ljubinka
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins // Poljoprivreda (Osijek), 17 (2011), 1; 23-27 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 517162 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins

Autori
Strelec, Ivica ; Has-Schön, Elizabeta ; Vitale, Ljubinka

Izvornik
Poljoprivreda (Osijek) (1330-7142) 17 (2011), 1; 23-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
barley variety discrimination ; gradient gel SDS-PAGE ; hordeins ; isoelectric focusing

Sažetak
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija



POVEZANOST RADA


Projekti:
113-1130473-0334 - Sinergističke smjese u antifungalnoj i antimikotoksigenoj zaštiti hrane (Klapec, Tomislav, MZOS ) ( CroRIS)
113-1130475-2722 - Unapređenje kakvoće, trajnosti i funkcionalnosti proizvoda na bazi pšenice (Koceva Komlenić, Daliborka, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prehrambeno-tehnološki fakultet, Osijek,
Sveučilište u Osijeku - Odjel za biologiju

Profili:

Avatar Url Ivica Strelec (autor)

Avatar Url Elizabeta Has-Schon (autor)

Avatar Url Ljubinka Vitale (autor)

Poveznice na cjeloviti tekst rada:

Hrčak

Citiraj ovu publikaciju:

Strelec, Ivica; Has-Schön, Elizabeta; Vitale, Ljubinka
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins // Poljoprivreda (Osijek), 17 (2011), 1; 23-27 (međunarodna recenzija, članak, znanstveni)
Strelec, I., Has-Schön, E. & Vitale, L. (2011) Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins. Poljoprivreda (Osijek), 17 (1), 23-27.
@article{article, author = {Strelec, Ivica and Has-Sch\"{o}n, Elizabeta and Vitale, Ljubinka}, year = {2011}, pages = {23-27}, keywords = {barley variety discrimination, gradient gel SDS-PAGE, hordeins, isoelectric focusing}, journal = {Poljoprivreda (Osijek)}, volume = {17}, number = {1}, issn = {1330-7142}, title = {Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins}, keyword = {barley variety discrimination, gradient gel SDS-PAGE, hordeins, isoelectric focusing} }
@article{article, author = {Strelec, Ivica and Has-Sch\"{o}n, Elizabeta and Vitale, Ljubinka}, year = {2011}, pages = {23-27}, keywords = {barley variety discrimination, gradient gel SDS-PAGE, hordeins, isoelectric focusing}, journal = {Poljoprivreda (Osijek)}, volume = {17}, number = {1}, issn = {1330-7142}, title = {Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins}, keyword = {barley variety discrimination, gradient gel SDS-PAGE, hordeins, isoelectric focusing} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)
  • Scopus


Uključenost u ostale bibliografske baze podataka::


  • AGRICOLA
  • CAB Abstracts





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