Pregled bibliografske jedinice broj: 517162
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins // Poljoprivreda (Osijek), 17 (2011), 1; 23-27 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 517162 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins
Autori
Strelec, Ivica ; Has-Schön, Elizabeta ; Vitale, Ljubinka
Izvornik
Poljoprivreda (Osijek) (1330-7142) 17
(2011), 1;
23-27
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
barley variety discrimination ; gradient gel SDS-PAGE ; hordeins ; isoelectric focusing
Sažetak
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija, Prehrambena tehnologija
POVEZANOST RADA
Projekti:
113-1130473-0334 - Sinergističke smjese u antifungalnoj i antimikotoksigenoj zaštiti hrane (Klapec, Tomislav, MZOS ) ( CroRIS)
113-1130475-2722 - Unapređenje kakvoće, trajnosti i funkcionalnosti proizvoda na bazi pšenice (Koceva Komlenić, Daliborka, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prehrambeno-tehnološki fakultet, Osijek,
Sveučilište u Osijeku - Odjel za biologiju
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Emerging Sources Citation Index (ESCI)
- Scopus
Uključenost u ostale bibliografske baze podataka::
- AGRICOLA
- CAB Abstracts