Naslov
Developmental patteerns of Ki-67, bacl-2 and caspase-3 proteins expression in the human upper jaw

Autori
Novaković, Josip ; Mardešić-Brakus, Snježana ; Vukojević, Katarina ; Saraga-Babić, Mirna

Izvornik
Acta Histochemica (0065-1281) 113 (2010), 5; 519-526

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Apoptosis; Ki-67; Bcl-2; Caspase-3; upper jaw development; human embryo

Sažetak
The distribution of the Ki-67, bcl-2 and caspase-3 proteins was immunohistochemically analyzed in the developing human upper jaw (5th–10th gestational weeks). During this period, proliferative activity gradually decreased from higher levels at the earliest stages (50–52%) to lower levels, both in the jaw ectomesenchyme and in the epithelium. The highest expression of bcl-2 protein was found in the epithelium and ectomesenchyme of areas displaying lower rates of cell proliferation. High levels of caspase-3 protein were detected during the earliest stages of jaw development, indicating an important role for apoptosis in morphogenesis of early derivatives of the maxillary prominences. The number of Ki-67, bcl-2 and caspase-3 positive cells changed in a temporally and spatially restricted manner, coincidently with upper jaw differentiation. While apoptosis might control cell number, bcl-2 could act in suppression of apoptosis and enhancement of cell differentiation. A fine balance between cell proliferation (Ki-67), death (caspase-3) and cell survival (bcl-2) characterized early human upper jaw development. A rise in the number of apoptotic cells always temporally coincided with the decrease in number of surviving bcl-2 positive cells within the palatal region. Therefore, the upper jaw development seems to be controlled by the precisely defined expression of genes for proliferation, apoptosis and cell survival.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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