Naslov
Synthesis of (di)adenosine polyphosphates by non-ribosomal peptide synthetases (NRPS)

Autori
Dieckmann, Ralf ; Pavela-Vrančić, Maja ; von Doehren, Hans

Izvornik
Biochimica et biophysica acta. Protein structure and molecular enzymology (0167-4838) 1546 (2001), 1; 234-241

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Peptide synthetase; Tyrocidine synthetase 1; (Di)nucleoside polyphosphate; (Di)adenosine tetraphosphate; Bacillus brevis

Sažetak
In response to nutritional stress conditions, Bacillus brevis produces the cyclodecapeptide antibiotic tyrocidine via tyrocidine synthetase, a multifunctional non-ribosomal peptide synthetase (NRPS). The apo-form of tyrocididne synthetase 1 forms adenosine (5')tetraphospho(5')adenosine, when incubated with MgATP2-, amino acid and inorganic pyrophosphatase. The synthesis is an intrinsic property of the adenylation domain, is strictly dependent upon the amino acid, and proceeds from a reverse reaction of adenylate formation involving a second ATP molecule. In the presence of tri- or tetrapolyphosphate preferential synthesis of adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate occurs, respectively. A potential involvement of adenosine (5')-n-phospho(5')adenosine in the regulation of the biosynthetic process has been suggested.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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