Naslov
Genetic variability of Croatian Citrus tristeza virus isolates

Autori
Černi, Silvija ; Nolasco, Gustavo ; Krajačić, Mladen ; Škorić, Dijana

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of 17th Conference of the International Organization of Citrus Virologist / - , 2010, 193-196

Skup
17th Conference of the International Organization of Citrus Virologist

Mjesto i datum
Adana, Turska, 22.10.2007. - 26.10.2007

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CP gene; CTV; phylogenetic analysis; SSCP

Sažetak
About 30 citrus field samples were collected from different orchards in the Croatian coastal region. The majority of trees was grafted on Poncirus trifoliata and displayed no symptoms of Citrus tristeza virus (CTV) infection. However, about 50% of tested trees were found CTV-infected. The genetic variability and population structure of CTV were studied by SSCP and sequence analysis of the cloned p25 (CP) and p23 genes. The results of phylogenetic analysis, corroborated by biological characterization, showed that both CP and p23 sequences clustered into phylogenetic groups that include sequences of severe quick decline, seedling yellows and stem pitting isolates. Evidence of mixed infections and recombination events was also found.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA