Naslov
Clinical Isolates of Pseudomonas aeruginosa - Drug resistance patterns and serovar determination

Autori
Škrobonja, Ivana ; Bubonja-Šonje, Marina ; Ambrožić, Damir ; Abram, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Knjiga sažetaka / - Zagreb, 2011, 53-53

Skup
9. hrvatski kongres kliničke mikrobiologije s međunarodnim sudjelovanjem

Mjesto i datum
Primošten, Hrvatska, 07.04.2011. - 09.04.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
pseudomonas aeruginosa ; antibiotic susceptibility ; serotype

Sažetak
Pseudomonas aeruginosa rods are opportunistic pathogens responsible generally for nosocomial infections. P. aeruginosa isolates were collected from hospitalized patients at University Hospital Rijeka, during 6 months period in 2009. The majority of examined strains were obtained from patients of the Intensive Care Units (62%) and were mostly isolated from endotracheal aspirates (73, 3%). Multiple samples from the same patient were counted as 1 if the same serotype was determined. Finally, 60 strains were serotyped by slide agglutination according to the International Antigenic Typing Scheme. Serotype O:11 was the most prevalent, accounting for approximately 47% of the isolates, followed by serotype O:1 (29%). Six isolates were the rare serotypes 0:7, 8, and 0:17, while 8, 3% of the isolates were nontypable or polyagglutinable. Antibiotic susceptibility of all isolates to common antipseudomonal antibiotics was determined by disk diffusion and E-test. Screening for metallo beta-lactamase (MBL) production was done by imipenem-EDTA MBL E-test. The induction of P. aeruginosa AmpC beta-lactamase by clavulanate, tazobactam, and cefoxitin was evaluated by the disk approximation methodology. All the isolates tested in the present study were multidrug-resistant. P. aeruginosa strains demonstrated resistance to meropenem (72%), gentamicin (76.7%), ciprofloxacin (71.7%), tobramycin (63.3%), amikacin (8.3%), piperacillin-tazobactam (6.7%), ceftazidime (5%). Six isolates (10%) were suspected to be MBL positive by the MBL E-test. 35% of the isolates had derepressed AmpC beta-lactamase activity. The PCR amplification method will be employed to identify strains that carried the MBL genes. For further epidemiological analysis, isolates will be examined for genetic relatedness by means of pulsed-field gel electrophoresis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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