Naslov
Optimiranje postupka razgradnje stanične stjenke svježeg pekarskog kvasca (Saccharomyces cerevisiae) metodom odzivnih površina
(Optimization the degradation of cell walls of fresh baker's yeast (Saccharomyces cerevisiae) by response surface methodology)

Autori
Đalić, Sanela

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Prehrambeno-tehnološki fakultet

Mjesto
Osijek

Datum
23.12

Godina
2010

Stranica
34

Mentor
Planinić, Mirela

Ključne riječi
ultrazvuk; ekstrakcija; Saccharomyces cerevisiae; proteini; metoda odzivnih površina
(ultrasound; extraction; Saccharomyces cerevisiae; proteins; resporse surface methodology)

Sažetak
The aim was to determine the optimal conditions for braking the cell walls of fresh baker's yeast (Saccharomyces cerevisiae) ultrasonic homogenization and optimization process by response surface methodology in order to release intracellular contents and that wehen it comes to protein denaturation. The influence of process parameters, cycle 0.4-0.7-1 with amplitude 40-70-100%, the ratio S/L from 0.3-0.8-1.3 g + 20 mL of buffer and time consuming process of breaking up cell walls 50-10-15 minutes, in relation to the total concentration of protein. RSM to approximate the conditions for protein extraction (Cycle 1, the amplitude of 100%, the ratio of S/L 0.03 (0, 6 g +20 mL buffer) and time of 15 minutes) at which the model provided a maximum yield of protein 17, 38 mg/gs.t.. To describe the extraction of total protein was successfully used linear model showed good agreement with experimental data, in addition to not reached the maximum function, means are needed further experimental research to achieve the optimal conditions for breaking the cell walls of yeast.

Izvorni jezik
Hrvatski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA