Naslov
Adsorption of BSA on Previously Formed Polyelectrolyte Multilayer ; Reflectometric and Electrokinetic Study

Autori
Đapić, Irena ; Kovačević, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Workshop of COST Action D43 Functionalized Materials and Interfaces : Book of Abstracts / - Madrid, 2011, 41-41

ISBN
978-84-694-2153-5

Skup
Workshop of COST Action D43 Functionalized Materials and Interfaces

Mjesto i datum
Madrid, Španjolska, 30.03.2011. - 01.04.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
adsorption ; electrokinetics ; polyelectrolyte multilayers

Sažetak
The methods of stagnation point optical reflectometry and electrophoretic light scattering were applied for investigation of adsorption of bovine serum albumin (BSA) on previously formed poly(allylamine hydrochloride)/poly(sodium 4-styrenesulphonate) (PAH/PSS) multilayer with PAH being a terminal layer [1, 2]. In the first case (optical reflectometry experiments) the solid substrate was silica in the form of silicon wafers carrying an oxide layer and in the latter one (electrokinetic experiments) silica particles. In both cases the build-up mechanism of PAH/PSS multilayers was examined with special emphasis on the effect of electrolyte concentration and on the influence of pH of solution on the multilayer formation process. Additionally, the effect of BSA concentration was investigated. It was shown that the adsorption of BSA depends on conditions under which the multilayer was formed (ionic strength, pH and presence of an anchoring layer), as well as on BSA concentration. It follows that adsorption of BSA could be controlled not only by choosing suitable BSA concentration, but also by modifying the preformed multilayer.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA