Naslov
AN AUTOMATED IMMUNOTURBIDIMETRIC ASSAY FOR HbA1c DETERMINATION

Autori
Vučić, Marijana ; Petrović, Sandra, Mesić, Ranko ; Ročić, Boris

Izvornik
Diabetologia Croatica (0351-0042) 27 (1998), 2; 85-90

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
HbA1c; immunoturbidimetric assay; automated procedure; diabetes mellitus

Sažetak
The aim of this study was to adapt a light-scattering competitive immunoassay for hemoglobin A1c (Unimate HbA1c, Roche Diagnostic Products, Hoffmann-La Roche, Basle, Switzerland) to an automated clinical chemistry analyzer (Olympus AU600, Olympus Optical Co., Tokyo, Japan). After hemolysis combined with enzymatic cleavage, hemoglobin A1c (HbA1c) and total hemoglobin were determined by immunoassay and colorimetry, respectively, and final result was calculated as a percentage from the two measured parameters ratio. The adapted procedure showed acceptable within- and between-assay imprecision (<5%) and an excellent agreement in comparison to ion-exchange chromatography (FPLC, Mono S column, Pharmacia, Uppsala, Sweden). Results from this study indicated the automated immunoassay to provide a precise, accurate and rapid tool for the routine determination of HbA1c.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA