Naslov
Altered activities of rat brain metabolic enzymes in electroconvulsive shock - induced seizures

Autori
Eraković, Vesna ; Župan, Gordana ; Varljen, Jadranka ; Laginja, Josip ; Simonić, Ante

Izvornik
Epilepsia (0013-9580) 42 (2001), 2; 181-189

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
electroconvulsive shock; glutamate dehydrogenase; aspartate transaminase; alanine transaminase; lactate dehydrogenase; alkaline phosphatase; creatinine kinase; gamma-glutamyltransferase

Sažetak
Purpose: Electroconvulsive shock (ECS) induces generalised seizure activity and provides an excellent experimental model for studying the effects of global electrical stimulation on various biochemical parameters. The aim of this work was to investigate the influence of a single or repeated ECS - induced seizures on rat brain metabolism. Methods: Experiments were carried out on female Hannover-Wistar rats divided into four groups: a) the control group, which was intact ; b) the 1 ECS group, which was killed 2 h after single ECS ; c) the 5 ECS group with 24 h rest, which was killed 24 h after the 5th daily ECS ; and d) the 10 ECS group with 48 h rest, which was given ECS every 48 h and killed 24 h after the 10th ECS. Activities of glutamate dehydrogenase (GLDH), aspartate-aminotransferase (AST), alanine-aminotransferase (ALT), gamma- glutamyltransferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and creatine kinase (CK) in the frontal cortex, cerebellum, hippocampus and pons-medulla region were determined. Results: Increased AST, ALP, LDH and CK activities were detected in all examined regions of the 1ECS and 5ECS groups. ALT activity was increased in the both mentioned groups except in the hippocampus of the 5ECS group, where increased GGT activity was detected. In the hippocampus of 1ECS group GLDH activity was decreased. Increased hippocampal AST and cortical CK activities, together with increased LDH activities in cortex, cerebellum and pons-medulla, were found. Conclusions: ECS-treatment induces region specific changes in metabolic activity- Neither a 24 h nor a 48 h rest period between two ECSs were sufficient for complete brain recovery, although most of the observed increased enzyme activities present in 1 ECS and 5 ECS were not present in 10 ECS.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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