Naslov
SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements

Autori
Nayler, O. ; Stratling, W. ; Bourquin, J.P. ; Štagljar, Igor ; Lindemann, L. ; Jasper, H. ; Hartmann, A.M. ; Fackerlmayer, F.O. ; Ullrich, A. ; Stamm, S.

Izvornik
Nucleic acids research (0305-1048) 26 (1998), 15; 3542-3549

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
Interphase chromatin is arranged into topologically separated domains comprising gene expression and replication units through genomic sequence elements, so-called MAR or SAR regions (from matrix- or scaffold-associating regions). S/MAR regions are located near the boundaries of actively transcribed genes and were shown to influence their activity. We show that scaffold attachment factor B (SAF-B), which specifically binds to S/MAR regions, interacts with RNA polymerase II (RNA pol II) and a subset of serine-/arginine-rich RNA processing factors (SR proteins). SAF-B localized to the nucleus in a speckled pattern that conicided with the distribution of the SR protein SC35. Furthermore, we show that overexpressed SAF-B induced an increase of the 10S splice product using an E1A reporter gene and repressed the activity of an S/MAR flanked CAT reporter gene construct in vivo. This indicates an association of SAF-B with SR proteins and components of the transcription machinery. Our results describe the coupling of a chromatin organizing S/MAR element with transcription and pre-mRNA processing components and we propose that SAF-B serves as a molecular base to assemble a "transcriptosome complex" in the vicinity of actively transcribed genes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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