Naslov
Concentration and purification of rubella virus using monolithic chromatographic support

Autori
Forčić, Dubravko ; Brgles, Marija ; Ivančić-Jelečki, Jelena ; Šantak, Maja ; Halassy, Beata ; Barut, Miloš ; Jug, Renata ; Markušić, Maja ; Štrancar, Aleš

Izvornik
Journal of chromatography. B (1570-0232) 879 (2011), 13/14; 981-986

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Rubella virus; virus purification; monoliths; chromatography; virus concentration

Sažetak
The production of economically acceptable viral vaccines of high quality requires simple and efficient methods for purification and concentration of viral particles. Ion-exchange chromatography (IEC) has become one of commonly used methods for large-scale downstream purification of viruses. Viruses possess different biological and/or biochemical properties and therefore IEC conditions must be established specifically for each virus. Live attenuated rubella virus vaccines have been manufactured and successfully used widely to protect people from rubella and congenital rubella syndrome for almost 40 years. The aim of this study was to search for an efficient method for concentration and purification of rubella virus using IEC. The selected operating conditions using quaternary amine monolithic supports enabled highly efficient binding, purification and concentration of rubella virus from complex biological suspension without additional procedures. Eluted viral particles maintained their infectivity and viral recovery was almost 100%. At the same time, viral preparation was successfully depleted from host cell protein and DNA. This work indicates the possibility of using monoliths to improve the rubella virus yields in productions where high virus titers during cultivation can hardly be achieved.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

POVEZANOST RADA