Pregled bibliografske jedinice broj: 50563
Regio- and stereoselectivity of biotransformation of steroid hormones by recombinant human CYP enzymes
Regio- and stereoselectivity of biotransformation of steroid hormones by recombinant human CYP enzymes // Drug Metabolism Reniews, Abstracts from the 10th North American ISSX Meeting / Hinson, JA (ur.).
New York (NY): Marcel Dekker, 2000. str. 251-251 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Regio- and stereoselectivity of biotransformation of steroid hormones by recombinant human CYP enzymes
Autori
Rendić, Slobodan ; Nolteernsting, Eckhard ; Schänzer, Wilhelm
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Drug Metabolism Reniews, Abstracts from the 10th North American ISSX Meeting
/ Hinson, JA - New York (NY) : Marcel Dekker, 2000, 251-251
Skup
10th North American ISSX Meeting
Mjesto i datum
Indianapolis (IN), Sjedinjene Američke Države, 24.10.2000. - 28.10.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
steroid hormones; CYP enzymes; biotransformation; stereoselectivity; regioselectivity; in vitro
Sažetak
Metabolism of isomers of endogenous steroid hormones was studied in vitro using human recombinant CYP3A4 and CYP2C19 enzymes. The enzyme formats used for CYP3A4 and CYP2C19 were insect cell microsomes expressing human CYP enzymes and purified recombinant human CYP enzymes in a reconstituted system. The CYP enzyme formats incubated with steroids, testosterone, epitestosterone, androstenedione, methyltestosterone, androsterone, 5-androstane-3,17-diol, 5-androstane-3,17-diol, 5-androstane-3,17-diol, 5-dihydrotestosterone (DHT), androst-4-ene-3,17-diol and androst-4-ene-3,17-diol , produced 6-hydroxyl metabolites identified as trimethylsilyl (TMS)-ethers by a gas chromatography-mass spectrometry
Izvorni jezik
Engleski
Znanstvena područja
Farmacija
