Naslov
Effect of olive oil enriched diet on fatty acid composition of brain phospholipids from mice during liver regeneration

Autori
Pantović, Radojka ; Tota, Marin ; Sigler, Melani ; Milin, Čedomila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXII. hrvatski skup kemičara i kemijskih inženjera / Croatian Meeting of Chemists and Chemical Engineers : Knjiga sažetaka / Book of Abstracts / Zagreb 2011 / - , 2011, 166-166

Skup
XXII. hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 13.02.2011. - 16.02.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
diet ; phospholipids ; fatty acids ; liver regeneration ; mice

Sažetak
Two groups of C57Bl mice, aged 8-10 weeks (5-8 animals per group) were fed, over 21 days, diets enriched with olive oil (5% addition to standard pellet, w/w), containing predominantly oleic acid (C18:1n-9), or standard pellet diet. The animals were one-third partially hepatectomised (1/3 pHx) under aether anaesthesia, sacrified by cervical disslocation 6th, 12th, 24th, and 48th hours later, when the brains were carefully removed. Total lipids were extracted from tissue samples with a chloroform/methanol (2:1, v/v) mixture according to Folch et al. Phospholipids (PL) were separated and purified from brain's total lipid mixture using solid phase extraction (SPE) procedure on aminopropylsilica columns. Fatty acids (FAs) of polar lipids were analysed in the form of fatty acid methyl esters (FAMEs) by gas chromatography (GC). Significant increases in the amounts of arachidonic and docosahexaenoic acids were detected in olive oil fed groups, 12 and 48 hours after pHx.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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