Naslov
Affinity capillary electrophoresis for the study of statin drug interactions with plasma proteins

Autori
Nigović, Biljana ; Sertić, Miranda ; Kujundžić, Nikola

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 3th International Conference on Drug Discovery & Therapy ; u: Current Medicinal Chemistry 18 (2011) S.I. / Atta-ur-Rahman - , 2011, 191-191

Skup
International Conference on Drug Discovery & Therapy (3 ; 2011)

Mjesto i datum
Dubai, Ujedinjeni Arapski Emirati, 07.02.2011. - 10.02.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
drug–protein interactions; affinity capillary electrophoresis; statin drugs; binding constants

Sažetak
An important topic in the discovery and development of new drugs is the role of drug binding to serum proteins. Drug–protein interactions are determining factors in the therapeutic and toxicological drug properties. Capillary electrophoresis has become a powerful tool in determining binding constants of formed drug-protein complexes. It offers some advantages in this kind of studies such as low sample requirement, short analysis time, high separation efficiency, sample throughput and low running costs. A new affinity capillary electrophoresis method was developed for the estimation of binding constant of statin drugs. In order to simulate physiological conditions, the electrophoresis was carried out using a phosphate buffer pH 7.4. Measurements were conducted at ten different protein concentrations added in the running buffer, while the drug was kept at the constant concentration in the sample. The apparent mobility of drug depends on the binding constant and the difference between the electrophoretic mobilities of free drug and drug-protein complex. Estimated values of binding constants were related with the structures of statins. The binding constants obtained by CE may help in early evaluation of drugs pharmacokinetic profiles during drug discovery.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA