Naslov
The influence of disposal technology obtained with alkaline treatments on D-amino acid content of slaughterhouse waste
(The influence of disposal technology obtained with alkaline treatments on D-amino acid content of slaughterhouse waste,)

Autori
Loki, Katalin ; Kalambura, Sanja ; Mandoki, Zsolt ; Varga-Vasi, Eva ; Albert, Csilla ; Csapo, Janos

Izvornik
Acta Universitatis Sapientiae Alimentaria (1844-7449) 3 (2010); 66-80

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, znanstveni rad, znanstveni

Ključne riječi
Alkaline treatment ; D-amino acids ; D-allo-isoleucine ; D-aspartic acid ; D-glutamic acid ; D-tryptophan ; racemization ; protein ; biological value
(Alkaline treatment ; D-amino acids ; D-allo-isoleucine ; D-aspartic acid ; D-glutamic acid ; D-tryptophan ; racemization ; protein ; biological value 6)

Sažetak
One possibility of disposing of animal proteins is alka- line hydrolysis that can be applied even for waste materials infected with transmissive encephalopathy since the alkaline hydrolysis decomposes the prions therefore it proved to be especially e®ective for the treatment of materials of animal origin of the highest risk. In our experiment the change in D-amino acid content of slaughterhouse waste due to the treat- ments was examined. The treatments were done with sodium and potas- sium hydroxide solution, respectively, for 2, 3 and 6 hours at 135, 150 and 153 ±C. D- Asp, D-Glu, and D-Trp content was determined using a Hitachi Merck LaChrom HPLC, and D-allo-Ile content using an INGOS amino acid analyzer. In case of tryptophan the hydrolysis was carried out with 3 M p-toluenesulfonic acid in the presence of 3-indolylpropionic acid, in other cases the 6 M hydrochloric acid hydrolysis was applied. Hydrolysis temperature was in both cases 110§2 ±C. Summarized, it can be said that due to the heat and alkali combinations we used aspartic acid, glutamic acid, tryptophan and isoleucine transform in 40-50% into the D-isomer. Even though the hydrolysed product obtained this way met in other parameters the requirements of the modern feeding, one should be expect that most of the amino acids undergo full racemization during this process.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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