Naslov
Different sugar beet pulp pretreatment methods for bioethanol production

Autori
Oros, Damir ; Vrgoč, Ivana ; Rezić, Tonči ; Šantek, Božidar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th International Congress of Food Technologists, Biotechnologists and Nutritionists / Božanić, Rajka i suradnici - Zagreb, 2011, 69-69

ISBN
978-953-99725-3-8

Skup
7th International Congress of FOOD TECHNOLOGISTS, BIOTECHNOLOGISTS AND NUTRITIONISTS

Mjesto i datum
Opatija, Hrvatska, 20.09.2011. - 23.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
beet pulp; bioethanol; ultrasound pretreatment; thermal pretreatment; enzymatic hydrolysis; stirred tank bioreactor

Sažetak
Sugar beet pulp (SBP) is a valuable lignocellulose’s waste raw material for bioethanol production. Bioethanol production process consists of following steps: lignocellulose pretreatment, fermentation and bioethanol separation and purification. Lignocellulose pretreatment is the main step for successful bioethanol production and wide investigation efforts were performed in order to develop the new efficient and cheap pretreatment method. Different physical, chemical and biological pretreatment methods could be used depending on the lignocellulose material source and composition. In this study, combination of physical and biological pretreatment was utilized in two consecutive steps. In the first step, thermal treatment or ultrasound disruption and in the second step enzymatic hydrolysis was used, respectively. For the enzyme hydrolysis commercial cellulases as well as combination of commercial cellulases and pectinases were utilized. Ultrasound disruption of SBP was carried out by ultrasound homogenisator SONOPLUS HD 2200 in continuous mode for 15 min with 50% of maximum power (max. 0.2 kW). Thermal treatment was performed at 121 OC for 15 min. Commercial cellulases (CN) and pectinases (PN) were used for the hydrolysis of SBP. Determination of hydrolyzed substrates and fermentation products was performed by high-pressure liquid chromatography (HPLC). It is obvious from obtained results that both pretreatment methods (thermal and ultrasound) of SBP have great potential for successful degradation of lignocellulose’s raw material. This observation can be most easily proved by the increase of mono– and disaccharide concentrations in cultivation medium. The extraction of remain sucrose (Suc) from SBP was considerably supported by the ultrasound treatment of SBP. Degradation of the lignocelluloses components of SBP to glucose (Glc) and galactose (Gal) was significantly increased by the thermal treatment. The treatment of SBP with celluloses and pectinases was related to the increase of glucose (Glc), galactose (Gal), arabinose (Ara) and galacturonic acid (GalA) concentrations. On the basis of obtained results it is clear that the combination of thermal treatment and enzyme hydrolysis is the most efficient SBP pretreatment method for bioethanol production.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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