Naslov
In vitro propagation of Dianthus gigantheus D'Urv. ssp. croaticus, a Croatian endemic plant species

Autori
Vujević, Marija ; Pevalek-Kozlina, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracta of the 12th Congress of the Federation of European Societies of Plant Physiology ; u: Plant Physiology and Biochemistry 38 (2000) (S) / Kader, Jean-Claude - Pariz : Elsevier, 2000, 26-26

Skup
Congress of the Federation of European Societies of Plant Physiology (12 ; 2000)

Mjesto i datum
Budimpešta, Mađarska, 21.08.2000. - 25.08.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dianthus gigantheus D'Urv. ssp. croaticus; endemic plant species; micropropagation

Sažetak
Dianthus gigantheus D'Urv. ssp. croaticus is a Croatian neoendemic plant species. Since the number of specimens has been evidently decreased due to ecological changes, destruction of natural habitats and human activity (Red Book of Plant Species, Republic of Croatia, 1994), the aim of this study was to establish the method for micropropagation of this endangered species. Seeds were collected on natural habitat. Sterilisation (Pevalek-Kozlina et al., 1997) was carried out with 2 percent Izosan-G (99 percent sodium dichloroisocyanurate dihydrate) for 5 min, and then, after three distilled water rinses (5 min each), with percent hydrogene peroxide (5 min). After three distilled water rinses (5 min each), seeds were inoculated on basal medium containing MS (Murashige and Skoog 1962) mineral salts, 0.1 g l–1 myo-inositol, 0.1 mg l–1 thiamine HCl, 0.5 mg l–1 pyridoxine HCl, 0.5 mg l–1 nicotinic acid, 30 g l–1 sucrose and 8 g l–1 agar. After four weeks, when seedlings reached lengths of 3–5 cm, shoots were separated from roots and transferred to the multiplication media. The multiplication of shoots was studied through four subcultures on basal medium containing 2.9 ľM gibberellic acid (GA3) and supplemented with different concentrations of 6-benzylaminopurine (BA) (0.1 ; 0.2 and 0.5 microM) or without BA. The multiplication rate was determined on 24 explants per medium and estimated after four weeks in culture. The average multiplication rate from four subcultures was calculated. Shoots excised from multiple shoot clusters were rooted on basal medium supplemented with different concentrations of indole-3-butyric acid (IBA) (2.5 ; 4.9 and 7.3 microM), indole-3-acetic acid (IAA) (5.7 ; 8.5 and 11.4 microM) and  -naphthalene acetic acid (NAA) (2.7 ; 5.4 and 8.1 microM) or without them. pH value of all media tested was adjusted to 5.8 before autoclaving. The cultures were incubated at 22ą2°C under a 16-hour photoperiod. All cultures remained sterile after four weeks in culture. Shoots originated from aseptically germinated seeds were used for culture initiation. Germination rate was 64.2 percent. The multiplication rate on medium without BA was 1.8 plants per explant. Addition of 0.1 and 0.2 microM BA slightly promoted the multiplication rate (2.0 and 2.1 plants per explant). The highest multiplication was achieved on medium with 0.5 microM BA (3.3). The results confirmed the promotive effect of BA on axillary bud proliferation (George and Sherrington, 1984). The inhibition of multiplication noticed in 2nd subculture in all media tested, except on medium with 0.1 microM BA, might be due to the seasonal changes. The rooting percentage was high on all media tested, with a slight suppression on media with higher concentrations of NAA. Both IBA and IAA resulted in 100 percent rooting. Rooting with IBA was faster and the roots were more branched.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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