Naslov
Using S. cerevisiae cell wall protein Ccw12p as a anchor for expression of two yeast nucleases at the cell surface

Autori
Teparić, Renata ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The secret life of biomolecules HBDMB2010 / Kovarik, Zrinka ; Varljen, Jadranka - Rijeka : Hrvatsko Društvo za Biotehnologiju, 2010

Skup
10th Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Opatija, Hrvatska, 15.09.2010. - 18.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yeast cell wall; Ccw12p; heterologous expression

Sažetak
In the past several years a number of surface – engineered yeasts, displaying different heterologous proteins interesting for biotechnological or medical applications, have been constructed. Yeast cell surface systems for the display of heterologous proteins have the advantages of simplicity of genetic manipulation, ability for proper post-translational modifications and folding of mammalian proteins and immobilization of heterologous proteins covalently under mild, physiological conditions without risk of damage by chemical treatment. S. cerevisiae cell wall proteins that are covalently bound to the carbohydrate components of the wall can be divided in two main groups. Majority of proteins of this class are bound at their C-termini through a remnant of the GPI-anchor. A smaller group of proteins are directly covalently bound at their N-termini to -1, 3-glucan by the alkali labile ester linkage between the glutamic acid γ-carboxyl group and hydroxyl groups of glucoses (Pir–proteins). In this work Ccw12p cell wall protein, that belongs to the group of cell wall proteins bound through GPI-anchor remnant, is used for C-terminal immobilization of two yeast intracellular nucleases, Yblp and Rnyp, to the yeast cell surface.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA