Naslov
Detection of heparin during F VIII isolation using improved azure A method

Autori
Gebauer, Branka ; Branović, Karmen ; Šimić, Mirjana ; Trešćec, Anđa ; Benko, Bojan

Izvornik
Acta pharmaceutica (1330-0075) 49 (1999), 1; 35-41

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
F VIII; heparin; azure

Sažetak
Cryoprecipitate with heparin was the starting material in chromatographic isolation of factor VIII (F VIII). Heparin stabilizes F VIII and in that sense enhances the recovery of F VIII during isolation. The heparin concentration during different steps of F VIII isolation procedure was determined by modified azure A method. Azure A method is a rapid and simple spectrometric method for determination of heparin following the formation of soluble complex between heparin and azure A dye. Influence of different parameters (pH, heparion concentration, stability of the dye and heparin-dye complex) on azure A method has been established. Results presented in this report show that determination of heparin is achieved by simultaneous measuring absorbance of the complex at 500 nm and azure A dye absorbance decrease at 630 nm.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA