Naslov
Differential properties of Grapevine virus B (GVB) isolates from Croatian autochthonous grapevine culitvars (Vitis vinifera L.)

Autori
Vončina, Darko ; Šimon, Silvio ; Đermić, Edyta ; Cvjetković, Bogdan ; Pejić, Ivan ; Maletić, Edi ; Karoglan Kontić, Jasminka

Izvornik
Journal of plant pathology (1125-4653) 93 (2011), 2; 283-289

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DASI-ELISA; RT-PCR; herbaceous hosts; sequencing

Sažetak
A total of 1116 grapevine accessions from 14 different Croatian autochthonous cultivars included in clonal selection were tested for the presence of Grapevine virus B (GVB) using antigen direct binding DASI-ELISA. Samples were taken from 51 commercial vineyards in the Dalmatian region. GVB was detected in 35 samples (3.14%) belonging to 9 different cultivars: Dobricic, Mladenka, Vlaška, Nincuša, Babic, Plavac mali, Maraština, Pošip and Vugava. The presence of GVB was further confirmed by molecular and biological assays. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) performed on 14 ELISA-positive samples using the primer pair H28/C410 confirmed the serological data. Partial nucleotide sequences for coat protein and putative RNA binding protein cistron were generated for 9 of these isolates and compared with each other as well as with other sequenced GVB isolates. Analyses showed sequence variation among Croatian GVB isolates: they shared 81.3-99.5% identical nucleotides and 79.8 – 83.9% with GVB reference isolate deposited in the GenBank. Deduced amino acid sequences of the part of the putative RNA-binding protein gene shared between 80.5 and 100% identity within Croatian isolates and 83.2 – 86.7% with reference isolate. Fourteen GVB-positive samples were selected for further biological characterization by mechanical inoculation onto a range of herbaceous hosts. Symptoms induced on herbaceous hosts varied according to the isolate. In most cases, GVB isolates from autochthonous Croatian cultivars induced chlorotic spots and/or vein banding in Nicotiana occidentalis and N. cavicola. However, in some cases, symptoms observed in these two virus hosts also included local necrotic lesions, yellowing, leaf deformation and leaf rolling/curling. To the best of our knowledge this is the first report of GVB in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA