Naslov
Development and characterization of the P- glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-reistant PLHC-1 hepatoma fish cell line

Autori
Lončar, Jovica ; Popovic, Marta ; Caminada, Daniel ; Fent, Karl ; Smital, Tvrtko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
TP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases: Book of Abstracts / - Innsbruck : Federation of European Biochemical Societies (FEBS), 2008, 131-131

Skup
2nd FEBS Special meeting: ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases

Mjesto i datum
Innsbruck, Austrija, 01.03.2008. - 08.03.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Pgp ; doxorubicine resistance ; PLHC-1

Sažetak
Similar to the MDR of mammals, aquatic organisms express Ppg1 and MRP-like proteins as important part of the multi-xenobiotic resistance (MXR) mechanism. Recently we demonstrated the presence of the ABC transport proteins in frequently used fish model, fish hepatoma cell line PLHC-1. In this study we selected highly resistant PLHC-1 cells (PLHC- 1/dox) by culturing them in the presence of doxorubicin. The 42 fold higher expression of ABCB1 gene was measured in PLHC-1/dox cells compared to non-selected cells (PLHC-1/wt). The efflux rates of model fluorescent Pgp1 substrates were 3-4 fold lower in PLHC-1/dox in comparison to PLHC-1/wt cells. Using MTT viability assay we showed that PLHC-1/dox are 45 fold more resistant to doxorubicin than PLHC-1/wt. Furthermore, similarly to mammalian cell lines, typical cross-resistance to other chemotherapeutics like daunorubicin, vincristine, vinblastine and colchicine, was developed. These data prove that specific, highly inducible Pgp1 mediated MXR mechanism is present in the PLHC-1 fish hepatoma cell line.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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