Pregled bibliografske jedinice broj: 500817
Tissue location as a determinant of T-cell survival following in vivo T-cell depleting treatment
Tissue location as a determinant of T-cell survival following in vivo T-cell depleting treatment // Book of abstracts Annual Meeting of the Austrian Society for Allergology and Immunology (OGAI)
Beč, Austrija, 2010. (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 500817 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Tissue location as a determinant of T-cell survival following in vivo T-cell depleting treatment
Autori
Mihalj, Martina ; Balogh, Peter
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts Annual Meeting of the Austrian Society for Allergology and Immunology (OGAI)
/ - , 2010
Skup
Annual Meeting of the Austrian Society for Allergology and Immunology (OGAI)
Mjesto i datum
Beč, Austrija, 03.12.2010. - 05.12.2010
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
secondary lymphoid organs; homeostatic proliferation; monoclonal antibody mediated T-cell depletion; memory T cell
Sažetak
T cell depletion is a widely used in transplanted patients to suppress rejection. Several studies with mice and humans showed that T cells with memory-like phenotype are relatively resistant to antibody-mediated T-cell depletion, but with no data on survival niches. Here we investigate the tissue characteristics where the resistant cells are spared from depletion during the early phase of treatment. We used IBL-1 rat monoclonal antibody against Thy-1/CD90 antigen in wild-type (BALB/c and [B6xBALB/c] F1) and transgenic mouse strains (c3-/-, AND TcR Tg). We found that in F1 mice in the blood and the spleen T-cells were almost completely eliminated, while in the peripheral lymph node (pLN) one third of the T-cells survived. In pLN samples we found significant (p=0, 009) increase of central memory (CD62L+/CD44hi) T cells. In AND mice with a large increase in memory T cells, 24 to 36 hours after the application of IBL-1 mAb we did not find any T-cell depletion in pLN’s compared to the spleen and blood. In contrast to F1 mice, both central and effector memory (CD62L-/CD44hi) T cells showed significant reduction. In BALB/c mice an extensive elimination of naïve T-cell and enrichment of memory cell type was observed in all tissue samples. However, pLN T cells showed significant resistance when compared with blood and spleen. Also, we found no difference in the frequency of regulatory T cells upon anti-Thy-1 treatment. Our results showed that pLNs in mice provide a protective environment for T-cell upon treatment with depleting antibodies, irrespectively of mouse strain, but with various capacities for protection.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
