Naslov
The replication of Legionella in alveolar epithelial cells

Autori
Ljubunčić, Samra ; Zoričić, Petra ; Rebić, Danica ; Gobin, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Liječnički vjesnik / - , 2010, 49-49

Skup
10th ZIMS, Zagreb International Medical Summit for students and young doctors

Mjesto i datum
Zagreb, Hrvatska, 11.11.2010. - 14.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Legionella; alveolar epithelial cells; replication; infection

Sažetak
Legionella genus comprise more than 50 species.In humans, these bacteria can cause Legionnaires´disease and Pontiac fewer. It has been proven that Legionella can multiply in alveolar macrophages. There are little information about proliferation of this bacteria in alveolar epithelial cells. Therefore, in this study we examined intracelullar growth of L. longbeachae and L. pneumophila in alveolar epithelial cell line. In this experiment L. longbeachae and L. pneumophila strain were used. Alveolar epithelial cell line (A549) cells was infected with bacteria at an MOI of 10. Bacterial replication was followed for three consecutive days after initial infection. Two hours after infection ~102 bacteria was isolated. After 72 hours there was an exponential growth of L. pneumophila (106 cfu/ml) and L. longbeachae (~105 cfu/ml). The intracellular localization of bacteria has been shown by Giemsa stain. The results indicate that both Legionella species have the ability to replicate in alveolar epithelial A549 cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA