ࡱ> 352bjbjVV .<<aaaaauuuu uj$b-aaa$QQQ:aaQQQQ huQ:0jQ%QaQl0"Q;j ':Use of genetic algorithm for optimization of fed-batch process of recombinant protein biosynthesis in bacteria Escherichia coli BL21+P Davor Valinger1, }elimir Kurtanjek1 1Laboratory for Measurement, Regulation and Automatisation, Faculty of Food Technology and Biotechnology, Zagreb, Croatia  HYPERLINK "mailto:dvalinger@pbf.hr" dvalinger@pbf.hr In this study genetic algorithm was used to optimize fed-batch process of recombinant protein biosynthesis. Genetic algorithm is commonly used to reduce number of experiments and to find an optimal result which in this case was highest yield of recombinant protein. To investigate glucose feeding rates on biosynthesis of recombinant protein in bacteria Escherichia coli BL21+P experiments were performed in 1,25 L bioreactors using bacteria E. coli transformed with expression vector coding for recombinant human protein. Batch and fed-batch experiments were performed with the same final amount of glucose added (15 g L-1). Results for fed-batch process were better in term of yield of recombinant protein, thus in this study optimization of fed-batch process was made. Using Luedeking-Piret model parameters for biosynthesis of recombinant protein were obtained and with genetic algorithm new mode   , . T V X Z \ ' ( M N O _ ` b } ~ ĸymy[mNmyF>FhomH sH h{`fmH sH h[hv0JmH sH #jh[hvUmH sH jhvUmH sH hvmH sH hvhvH*mH sH hZH*mH sH hOhOH*mH sH hOmH sH h'RhO>*H*mH sH h'RhO>*mH sH h?AhZ0J]mH sH hOhZ0J5]mH sH !hOhZ0J56]mH sH hOhZ5mH sH   X Z ' a b m $[$\$]a$gd!`gdo$[$\$]a$gdZ[$\$]gdO F N  # $ l m , : L d e f %&'P[\a}վ𮦮ՒՒՒՒUhB"mH sH hV<hV<H*mH sH h emH sH hV<mH sH hcmH sH h{ChomH sH h m$ho6mH sH homH sH h{Ch m$mH sH h m$mH sH h8UmH sH h^mH sH 6l for fed-batch process was developed. New optimized model of fed-batch process obtained by genetic algorithm showed increase of yield of recombinant protein by 10 % compared to best fed-batch experiment. ,1h. A!"#$% DyK dvalinger@pbf.hryK 0mailto:dvalinger@pbf.hr^ 2 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~_HmHnHsHtH@`@ NormalCJ_HaJmHsHtHDA`D Default Paragraph FontRiR  Table Normal4 l4a (k (No List <B@< !` Body Textdd[$\$,O, Z yshortcuts4U@4 v Hyperlink >*phPK![Content_Types].xmlj0Eжr(΢Iw},-j4 wP-t#bΙ{UTU^hd}㨫)*1P' ^W0)T9<l#$yi};~@(Hu* Dנz/0ǰ $ X3aZ,D0j~3߶b~i>3\`?/[G\!-Rk.sԻ..a濭?PK!֧6 _rels/.relsj0 }Q%v/C/}(h"O = C?hv=Ʌ%[xp{۵_Pѣ<1H0ORBdJE4b$q_6LR7`0̞O,En7Lib/SeеPK!kytheme/theme/themeManager.xml M @}w7c(EbˮCAǠҟ7՛K Y, e.|,H,lxɴIsQ}#Ր ֵ+!,^$j=GW)E+& 8PK!Ptheme/theme/theme1.xmlYOo6w toc'vuر-MniP@I}úama[إ4:lЯGRX^6؊>$ !)O^rC$y@/yH*񄴽)޵߻UDb`}"qۋJחX^)I`nEp)liV[]1M<OP6r=zgbIguSebORD۫qu gZo~ٺlAplxpT0+[}`jzAV2Fi@qv֬5\|ʜ̭NleXdsjcs7f W+Ն7`g ȘJj|h(KD- dXiJ؇(x$( :;˹! I_TS 1?E??ZBΪmU/?~xY'y5g&΋/ɋ>GMGeD3Vq%'#q$8K)fw9:ĵ x}rxwr:\TZaG*y8IjbRc|XŻǿI u3KGnD1NIBs RuK>V.EL+M2#'fi ~V vl{u8zH *:(W☕ ~JTe\O*tHGHY}KNP*ݾ˦TѼ9/#A7qZ$*c?qUnwN%Oi4 =3ڗP 1Pm \\9Mؓ2aD];Yt\[x]}Wr|]g- eW )6-rCSj id DЇAΜIqbJ#x꺃 6k#ASh&ʌt(Q%p%m&]caSl=X\P1Mh9MVdDAaVB[݈fJíP|8 քAV^f Hn- "d>znNJ ة>b&2vKyϼD:,AGm\nziÙ.uχYC6OMf3or$5NHT[XF64T,ќM0E)`#5XY`פ;%1U٥m;R>QD DcpU'&LE/pm%]8firS4d 7y\`JnίI R3U~7+׸#m qBiDi*L69mY&iHE=(K&N!V.KeLDĕ{D vEꦚdeNƟe(MN9ߜR6&3(a/DUz<{ˊYȳV)9Z[4^n5!J?Q3eBoCM m<.vpIYfZY_p[=al-Y}Nc͙ŋ4vfavl'SA8|*u{-ߟ0%M07%<ҍPK! ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э5 6?$Q ,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 +_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!Ptheme/theme/theme1.xmlPK-! ѐ' theme/theme/_rels/themeManager.xml.relsPK]    'N_X8@0(  B S  ?3?j3>j3D>j&&9*urn:schemas-microsoft-com:office:smarttagsplace8*urn:schemas-microsoft-com:office:smarttagsCityB*urn:schemas-microsoft-com:office:smarttagscountry-region p'`l{3b'`ZB" m$&Fl,V<]aA8U!`{`f\vfYc^ eOo'Rv$c@bAbb(``@`(@UnknownG*Ax Times New Roman5Symbol3. *Cx ArialACambria Math"1SFSF  !24d3qHP ?]aA2!xx`In this study genetic algorythm was used to optimize fed batch production of recombinant proteinxMajaOh+'0x $ D P \hpxdIn this study genetic algorythm was used to optimize fed batch production of recombinant proteinxNormalMaja2Microsoft Office Word@0@2h@2h՜.+,D՜.+,H hp|  )xx  aIn this study genetic algorythm was used to optimize fed batch production of recombinant protein Title 8@ _PID_HLINKSAhWemailto:dvalinger@pbf.hr  !#$%&'()+,-./014Root Entry Fh6Data  1TableWordDocument.SummaryInformation("DocumentSummaryInformation8*CompObjy  F'Microsoft Office Word 97-2003 Document MSWordDocWord.Document.89q