Naslov
Cytotoxic and apoptotic effects of 17α-ethynylestradiol and diethylstilbestrol on CHO-K1 cells

Autori
Radošević, Kristina ; Novak, Ruđer ; Slivac, Igor ; Mihajlović, Mirna ; Dumić, Jerka ; Kniewald, Zlatko ; Gaurina Srček, Višnja

Izvornik
Food technology and biotechnology (1330-9862) 49 (2011), 4; 447-452

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
apoptosis; CHO-K1 cells; cytotoxicity; diethylstilbestrol; 17α-ethynylestradiol; necrosis

Sažetak
There is considerable concern about the substances present in the environment and their potential to interfere with the endocrine system of vertebrates. Among these so-called endocrine-disrupting compounds, which can modulate or disrupt developmental and reproductive processes, substances with estrogenic activity have attracted most attention. Concerns about the presence of those compounds in the environment have led to the development of screening and testing assays that are able to detect such substances and evaluate their potential to induce adverse effects. In vitro systems such as mammalian and fish cell lines have become of growing importance in toxicity testing of such compounds. The cytotoxic and apoptotic effects induced by 17α-ethynylestradiol and diethylstilbestrol were studied on CHO-K1 cell line. Trypan blue exclusion method was used to determine the cell viability. Cytotoxicity of 17α-ethynylestradiol (0.34-34 µM) and diethylstilbestrol (0.37-37 µM) was found to be concentration-dependent with IC50 values of 12.8 µM and 10.4 µM after 72 h exposure, respectively. In treated CHO-K1 culture cell death was assessed by determing morphological changes by hematoxilyn-eosin staining, nuclear morphology and cell viability by fluorescein diacetate/propidium iodide staining and fluorescence microscopy, DNA fragmentation by TUNEL method and translocation of phosphatidyl serine by flow cytometry. Obtained results showed that 17α-ethynylestradiol induced apoptosis, while diethylstilbestrol induced necrosis in treated CHO-K1 cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA