Naslov
Virulence comparison of human and poultry Campylobacter jejuni isolates in a mouse model

Autori
Vučković, Darinka ; Wraber, Branka ; Abram, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes, Programme and Abstracts / Fece, Jadranka ; Kos, Blaženka ; Mrša, Vladimir - Zagreb : Hrvatsko mikrobiološko društvo, 2010, 144-144

ISBN
978-953-7778-00-2

Skup
Power of Microbes in Industry and Environment 2010.

Mjesto i datum
Malinska, Hrvatska, 22.09.2010. - 25.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Virulence; Campylobacter; mouse model

Sažetak
In this study we used previously established experimental model of systemic C. jejuni infection in mice suitable for pathogenesis and immunity investigations. Intravenous challenge of BALB/c mice was used to compare the relative virulence of two different strains of C. jejuni: one human clinical isolate and one poultry derived isolate. The infected mice were followed for eight days post-challenge and the bacterial numbers, presence/absence of lesions and localized or generalized nature of the disease were assessed. At the same time plasma was collected for IL-10 and IFN- γ determination. The infection achieved by both bacterial strains was systemic as campylobacters were isolated from different organ homogenates during the whole observed period post-infection. However, Campylobacter spp. exhibited strain differences in virulence according to different numbers of bacteria recovered with a trend towards the isolate originating from poultry. The pronounced difference in inducing cytokine production between the two strains was seen in the case of IL-10 where the concentration was higher in plasmas originating from mice infected with C. jejuni clinical isolate. Thus, lower production of IL-10 in mice infected with poultry derived isolate was associated with increased local inflammation and systemic morbidity, as well as higher bacterial burden.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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