Naslov
The immunoevasive function encoded by the mouse cytomegalovirus gene m152 protects the virus against both the adaptive and innate immune response

Autori
Krmpotić, Astrid ; Messerle, Martin ; Crnković-Mertens, Irena ; Bubić, Ivan ; Hengel, Hartmut ; Jonjić, Stipan ; Koszinowski, Ulrich

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian Immunological Society / Rabatić, Sabina - Zagreb : Hrvatsko imunološko društvo, 1999

Skup
Annual meeting of the Croatian Immunological Society

Mjesto i datum
Zagreb, Hrvatska, 25.11.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytomegalovirus; m152 gene; T cells; NK cells

Sažetak
Cytomegaloviruses encode numerous products that inhibit antigen presentation in the MHC class I pathway in vitro. The mouse cytomegalovirus (MCMV) glycoprotein gp40, encoded by the m152 gene retains murine MHC class I complexes in the ERGIC/cis-Golgi compartment (Ziegler et al., Immunity 6:57, 1997). To investigate the in vivo significance of this gene function during MCMV infection of the natural host, we constructed recombinants of MCMV in which the m152 gene was deleted, as well as the corresponding virus revertants. Deletion of the m152 gene has no effect on virus replication in cell culture, whereas after infection of mice the m152 deficient virus replicates to significantly lower virus titers. This attenuating effect is lifted by reinsertion of the gene into the mutant. Mutants and revertants grow to the same titer in mice deficient for MHC-class I effector function. These results prove that MHC reactive functions protect cytomegaloviruses against the attack by CD8+ T lymphocytes in vivo (Krmpotić et al., J. Exp. Med. 190:1285, 1999). In addition, the m152 deletion affects virus control by NK cells in vivo. However, contrary to the expected, the deletion of the m152 function, although associates with increased MHC class I expression, renders the virus more sensitive to NK cells. This NK cell sensitizing effect is mouse strain specific. Since infected cells from responder and nonresponder strains are recognized, specificity is defined at the level of the NK cell receptor. In conclusion, we showed for the first time that a herpesvirus protein can downmodulate both the adaptive and the innate immune.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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