Naslov
Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol

Autori
Pavlica, Mirjana ; Klobučar, Goran Igor Vinko ; Vetma, Nataša ; Erben, Radovan ; Papeš, Dražena

Izvornik
Mutation Research (1383-5718) 465 (2000); 145-150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Dreissena polymorpha; Planorbarius corneus; micronucleus assay; haemocytes; PCP

Sažetak
The frequency of micronuclei (MN) induced by pentachlorophenol (PCP) in haemocytos of zebra mussel, Dreissena polymorpha Pall. and great ramshorn snail, Planorbarius corneus L. was determined over a 14 days of exposure (sampling after 4, 7 and 14 days) under laboratory conditions. PCP doses for zebra mussel ranged from 10 to 150 mg/l, and for ramshorn snail form 10 to 450 mg/l. Micronuclei were detected after bisbenzimide fluorescent staining. Positive responses were observed in both species. The mean MN frequencies in treated mussels ranged between 0.69 and 7.50%o, and between 2.07 and 13.80%o in treated snail. The spontaneous MN levels in mussels averaged from 0.5 to 2.75%o, and in snails from 1.56 to 2.00%o. Our results suggest that haemolymph of both species represent an appropriate test tissue in environmental genotoxicity assessment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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