Naslov
E-cadherin in the central nervous system tumors meningiomas

Autori
Pećina-Šlaus, Nives ; Nikuševa Martić, Tamara Zeljko, Martina ; Deak, Adam Jakov ; Hrašćan, Reno ; Tomas Davor ; Musani, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDIR-1 From Bench to clinic / Sabol, Maja ; Levanat, Sonja - Zagreb : Institut Ruđer Bošković, 2010

Skup
HDIR-1 From Bench to clinic, Hrvatsko društvo za istraživanje raka

Mjesto i datum
Zagreb, Hrvatska, 11.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
E-cadherin; CDH1; loss of heterozygosity; immunostaining; meningiomas; tumors of the CNS; wnt signaling pathway

Sažetak
The molecular mechanisms and candidate genes involved in development of meningiomas still need investigation and elucidation. In the present study thirty-three meningiomas were analyzed regarding genetic changes of tumor suppressor gene E-cadherin (CDH1), a component of adherens junction and an indirect modulator of the wnt signaling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) method. Protein expression was analysed by immunohistochemistry. The results of our analysis showed altogether 32% of samples with LOH of the CDH1 gene. Interestingly, another type of genomic instability was detected replication error-positive samples (RER+). Three out of 28 heterozygous samples were RER-positive (11%). The instability is the result of impaired cellular mismatch repair. Fibrous and angiomatous cases showed higher percent of genetic changes, 67% and 75%, respecitively. Immunostaining showed that 58% of samples had downregulation of E-cadherin expression. Five out of nine samples with LOH were accompanied with the downregulation of E-cadherin protein expression (56%). One RER+ sample had lower expression of E-cadherin. We noticed that 37% of samples with lower E-cadherin expression had beta-catenin located in the nucleus. Also, 75% of samples with genomic instabilities had beta-catenin in the nucleus. Our findings demonstrated that there is significant association between the genetic changes of CDH1 and the nuclear localization of beta-catenin protein (2 =5, 25, df =1, P0, 022). Our results suggest that microsatellite genetic instabilities of the E-cadherin gene have a role in meningioma development and progression. Detected instability indicates that mismatch repair may also be targeted in meningioma.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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