Naslov
Comparative protein analysis of sugar beet cell lines by two-dimensional electrophoresis

Autori
Krsnik-Rasol, Marijana, Cipcic, Hana ; Poljuha, Danijela ; Hagege, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Actes du 12eme Colloque de Biotechnocentre / Gauthier F. - Seillac : Conseil Regional du Centre, 1999

Skup
12eme Colloque du Biotechnocentre

Mjesto i datum
Seillac, Francuska, 04.11.1999. - 05.11.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sugar beet cell lines; 2-D electrophoresis; habituation; tumourogenesis
(beet cell lines; 2-D electrophoresis; habituation; tumourogenesis)

Sažetak
Although the number of proteins identified from two-dimensional gels is often only a small percentage of the total proteome, the method is helpful when complex samples of proteins need to be analysed. The purpose of our sudy was to compare four sugar beet (Beta vulgaris L.) cell lines with the regaard to their protein expression. The following lines were analysed: N -normal hormone dependent, HO - habituated, producing shoots , HNO - habituated unorganised, T - crown-gall-tumour line. Preliminary results showed qualitative and quantitative differences in protein spots of different cell lines. The majority of total soluble proteins were detected in the pH range between 5 and 8. Tumour expressed more proteins in the neutral region of the gel, while the other lines had more spots in the acidic region.

Izvorni jezik
Engleski

POVEZANOST RADA