Pregled bibliografske jedinice broj: 491227
In vitro effect of the antimalarial drug proguanil hydrochloride on viability and DNA damage in human peripheral blood lymphocytes
In vitro effect of the antimalarial drug proguanil hydrochloride on viability and DNA damage in human peripheral blood lymphocytes // Environmental toxicology and pharmacology, 30 (2010), 3; 257-263 doi:10.1016/j.etap.2010.07.001 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 491227 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
In vitro effect of the antimalarial drug proguanil hydrochloride on viability and DNA damage in human peripheral blood lymphocytes
Autori
Gajski, Goran ; Dinter, Domagoj ; Garaj-Vrhovac, Vera
Izvornik
Environmental toxicology and pharmacology (1382-6689) 30
(2010), 3;
257-263
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
proguanil ; cycloguanil ; human lymphocytes ; genotoxicity ; comet assay ; sister chromatid exchange analysis
Sažetak
This study aimed to evaluate the effect of proguanil, a chemical substance used for treatment and prevention of malaria on viability and DNA integrity in human lymphocytes in vitro. Two different concentrations of proguanil obtained from the plasma concentrations were used: 130. ng/ml used for prophylactic treatment and 520. ng/ml used in treatment of malaria. Testing was done with and without metabolic activation. Viability of lymphocytes decreased in time and dose dependent manner. Comet assay parameters showed similar effects, indicating that some damage to DNA molecule can occur. Frequency of sister chromatid exchanges did not show significant deviation from the control samples. As for the proliferation kinetics no significant changes were noticed. Since majority of DNA damaging effect is induced after metabolic activation it is to be concluded that activity of proguanil is dependent upon the active metabolite cycloguanil and that monitoring should be conducted especially among frequent travellers.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Farmacija
POVEZANOST RADA
Projekti:
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Garaj-Vrhovac, Vera, MZOS ) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
PLIVA HRVATSKA d.o.o.
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
