Naslov
Extracellular glycoproteins in embryogenic cell culture of pumpkin (Cucurbita pepo L.)

Autori
Čipčić, Hana ; Leljak, Dunja ; Krsnik-Rasol, Marijana ; Hagege, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12eme Colloque de Biotechnocentre : resumes ; u: Biology of the Cell 92 (2000) (S) / Gauthier, F. - Seillac : Conseil Regional du Centre, 2000, 6-6

Skup
Colloque de Biotechnocentre (12 ; 1999)

Mjesto i datum
Seillac, Francuska, 04.11.1999. - 05.11.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
embryogenesis; Cucurbita pepo; extracellular glycoproteins; tunicamycin

Sažetak
To uderstand the molecular events controlling embryogenesis, it is helpful to identify the expression patterns of genes and proteins playing key roles in the progress of developmental pathways. We analysed extracellular glycoprotein patterns in pumpkin embryogenic tissue lines to detect some developmentally spscific glycoproteins. The influnce of nitrogen salts, auxin an tunicamycin on embryo development was tested ond different media. All lines excreted the glycoproteins of 35 and 37.5 kDa. The 66.5 kDa glycoprotein was expressed in the DEC and HEC lines. Its disappearance after tunicamycin treatment didi not cause the loss of embryogenic potential. Glycoproteins of 47 and 78 kDa deteced in the DEC and HEC might be involved in embryo development to mature stages.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA