Naslov
The intestinal factor Tff3 and a miRNA network regulate murine caloric metabolism

Autori
Shah, Aftab Ali ; Leidinger, Petra ; Keller, Andreas ; Wendschlag, Anke ; Backes, Christina ; Baus Lončar, Mirela ; Meese, Echart and Blin, Nikolaus

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EMBO|EMBL Symposium: Non-Coding Genome: Book of Abstracts / - , 2010

Skup
EMBO|EMBL Symposium: Non-Coding Genome

Mjesto i datum
Heidelberg, Njemačka, 13.10.2010. - 16.10.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dietary metabolism; trefoil peptides; knock-out mouse; transcriptional profiling; microRNA

Sažetak
Gene impairment of the genes for three mammalian trefoil peptides resulted in severe gastrointestinal malfunctions. The trefoil peptides also appear involved in caloric metabolism. Monitoring global miRNA expression of Tff3 deficient mice points to an interplay of Tff3 with a miRNA regulatory network. We identified 21 miRNAs that were deregulated when compared to the wild type strain. In silico evaluation indicated that the majority of the 21 miRNA were connected with the metabolic pathway "glycolysis/gluconeogenesis' (p=0.032), a signaling pathway including nine target genes Aldh9a1, Aldh2, Pck1, Aldoc, Pgam2, Pck2, Adh4, Adh5, and Fbp1. Association of Tff3 with this metabolic pathway is further supported by the observation that the body mass of adult Tff3 KO mice (five months) showed a clearly reduced weight. Furthermore, the majority of the identified 21 miRNA genes are localized on murine chromosomes 2 and 5 in three clusters (2A1, 2B, 5B3) suggesting a coordinated expression control and function.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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