Naslov
Reversal of Multidrug Resistance in Murine Lymphoma Cells by Amphiphilic Dihydropyridine Antioxidant Derivative

Autori
Cindrić, Marina ; Čipak, Ana ; Serly, Julianna ; Plotniece, Aiva ; Jaganjac, Morana ; Mrakovčić, Lidija ; Lovaković, Tomislava ; Dedić, Azra ; Soldo, Ivo ; Duburs, Gunars ; Žarković, Neven ; Molnár, József

Izvornik
Anticancer research (0250-7005) 30 (2010), 10; 4063-4070

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cancer ; doxorubicin ; MDR reversal ; dihydropyridine derivative ; oxidative stress ; antioxidant

Sažetak
Multidrug resistance, the principal mechanism by which cancer cells develop resistance to chemotherapy drugs, is a major factor in the failure of many forms of chemotherapies. The aim of the study was to investigate the effect of K-2-11 on the reversal of multidrug resistance. The effects of amphiphilic dihydropyridine derivative K-2-11 were tested on MDR1-expressing mouse lymphoma cells and their parental control. The effects of K-2-11 with and without doxorubicin were studied by determination of cell viability, cell proliferation and production of reactive oxygen species. K-2-11 caused complete reversal of multidrug resistance of the MDR cells, being much more efficient than the positive control verapamil. Accordingly, the cytotoxic effects of doxorubicin were enhanced by K-2-11, both in the MDR and in parental cell line, while K-2-11 alone did not affect cell viability. K-2-11 also acted as an antioxidant, reducing the cellular generation of reactive oxygen species. Our results indicate the high potential of K-2-11 as a novel antioxidant with potent MDR-blocking ability that should be studied further for development in adjuvant anticancer treatments.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA