Naslov
An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts

Autori
Novak Kujundžić, Renata ; Ragland, William Lauman

Izvornik
Acta veterinaria (Brno) (0001-7213) 79 (2010), 3; 431-436

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mRNA ; absolute abundance ; poly I:C

Sažetak
Immunosuppression of commercial chickens is a serious animal health and economic problem in the poultry industry. The major causes of the immunosuppression are viruses that suppress transcription of interferon genes, especially interferon alpha. There is a need for monitoring of immunosuppression in commercially bred chickens. For this purpose, the absolute abundance of interferon alpha transcripts can be measured in blood of chickens by a suitable assay. Such an assay was used to estimate abundance of chicken interferon alpha in a sample of splenic cells induced with polyinosinic polycytidylic acid. The abundance measured was 29 ± 2 attomoles/µg total RNA. This assay can be performed in microtitre plates using samples collected from chickens in poultry houses.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA