Naslov
Correlation of Ikaros Family Members Expression and the Occurrence of Human Lymphoma

Autori
Antica, Mariastefania ; Dubravčić, Klara ; Raić, Ljubica ; Labar, Boris ; Batinić, Drago

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytometry Part A / Tárnok, Attila - , 2007

Skup
11. Leipziger Workshop, 4th International Workshop Slide Based Cytometry “Cytomics and the Human Cytome Project”

Mjesto i datum
Leipzig, Njemačka, 27.04.2006. - 29.04.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Ikaros ; lymphoma ; lymphoocytes ; flow cytometry

Sažetak
Transcription factors from the Ikaros family, Aiolos and Helios play an essential role in the commitment of hematopoietic progenitors into the lymphoid lineage as well as in the choice of effector functions at later stages of development. Gene targeting studies in mice and also studies of cell populations from leukemic patients indicate that Ikaros, Aiolos and Helios might be involved in leukemogenesis. Therefore we analyzed Aiolos and Helios expression in 8 hematological cell lines, and leukemic cells from 17 patients with morbus Hodgkin's and 24 patients with non- Hodgkin's lymphoma. We used reverse transcription- PCR on RNA isolated from formalin fixed and paraffin embedded lymph node tissues previously evaluated by pathohistological and immunophenotypic criteria. We also analyzed protein expression in leukemic cell subpopulations. Our results show that the pattern of expression of Aiolos and Helios is not homogeneous among different subsets of leukemia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA