Naslov
Increased beta-defensin levels in airways of RSV infected infants

Autori
Bendelja, Krešo ; Roglić, Srđan ; Vojvoda, Valerija ; Tešović, Goran ; Mlinarić-Galinović, Gordana ; Rabatić, Sabina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2010 Annual Meeting of the Croatian Immunological Society, Book of Abstracts / - , 2010

Skup
2010 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Mali Lošinj, Hrvatska, 23.09.2010. - 26.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSV; beta-defensin; infants; BAL

Sažetak
Respiratory syncytial virus (RSV) causes acute respiratory tract illness across all ages while lower respiratory tract infection (LRTI) is usually a result of primary infection in infants. Supportive care is the mainstay of therapy and includes oxygenotherapy, use of bronchodilators and corticosteroids, although no convincing data exist for their efficacy. Antimicrobial innate defense along with transferred maternal immunoglobulins play a significant role in infants while immune system undergoes conditional maturation. Beta- defensins are small antimicrobial peptides produced by epithelial cells and mononuclear leukocytes with wide pathogen specificity, displaying neutralization/inactivation capability. Even more, premature newborns show lower beta- defensin 1 production that might compromise early innate response. We determined levels of beta-defensin 1 and 2, IL- 1beta, TNF-alpha and IL-6 in BAL samples from infants with RSV-caused LRTI and infants with bacterial pneumonia in order to determine a profile of secreted antimicrobials. Infants admitted to ICU with confirmed RSV- infection (N=13) and infants with bacterial pneumonia (N=8) underwent to bronchoscopy. Infants with human metapneumo virus (HMPV) infection (N=3) were also included. High levels of both beta- defensins were detectable in majority of infants with higher levels in infants with acute RSV infection compared to infants with bacterial pneumonia and HMPV infection. Beta-defensin levels correlated with levels of pro-inflammatory TNF- alpha and IL- 1beta. Although, RSV and bacteria could both stimulate secretion of beta-defensins via pro- inflammatory cytokine loop, higher levels of beta- defensins 1 and 2 in acute RSV infection. Sustained high level of antimicrobial production by RSV challenged epithelial cells and infiltrated leukocytes could augment local inflammation and emphasize immunopathology seen in RSV infection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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