Naslov
Hematological changes in piglets undergoing splenectomy and autologous spleen transplantation

Autori
Poljičak-Milas, Nina ; Vujnović, Anja ; Vnuk, Dražen ; Milinković-Tur, Suzana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
First European Joint Congress of EFCC and UEMS "Laboratory Medicine in Health Care, Laboratory Medicine at the Clinical Interface" : Abstracts Book / Carvalho, Rodrigues - Lisabon, 2010, 172-172

Skup
European Joint Congress of EFCC and UEMS (1 ; 2010)

Mjesto i datum
Lisabon, Portugal, 13.10.2010. - 16.10.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hematology; piglets; splenectomy; autotransplantation

Sažetak
Many hematological changes have been noted in the peripheral blood following removal of the spleen, but there are diverse reports of certain hematological parameters in various species. When total splenectomy is unavoidable, spleen autotransplantation can be performed in order to restore the function of the intact spleen, however, there is a still controversy about effectiveness of transplanted splenic tissue. The study was conducted on nineteen Landras piglets aged three months. Piglets were randomly divided in to three groups: sham-operation (n=6), total splenectomy (n=6), and splenic autotransplantation (n=7) with spleen chips autotransplanted into the greater omentum. The blood samples were taken just before the surgery, and on 1st, 5th, 12th, 26th and 40th postoperative day. EDTA blood samples were used to determine complete blood count using hematological counter SERONO – 9120 Baker System. White blood cell differentiation and reticulocyte count was performed microscopically on blood smears stained with May-Grünwald-Giemsa method, and methylene blue vital dye. Postoperative erythrocyte, hemoglobin and hematocrit depressions were found in all groups up to the 12th day of experiment. After that, erythrocyte parameters recovery was noted only in the control group. Reticulocyte number was increased during whole experiment in the splenectomized and autotransplanted group ranged from 1.5 to 10 times the preprocedure level, subsequent with sporadic finding of circulating normoblasts, and appearance of Howell-Yolly bodies. In control piglets, marked reticulocytosis was diminished at 40th postoperative day. Platelet number ranged between reference values in all investigated piglets. Following the operation, all groups showed leukocytosis with an increase of the absolute neutrophil number and initial decrease of the lymphocyte number which was followed by the later recovery. Noted leukocytosis was accompanied with increased band neutrophil number up to 12th postoperative day. At the 12th postoperative day reactive reactive lymphocytes were frequently found in the autotransplantated group. These results are widening the knowledge about hematological effects of splenectomy and splenic implants.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA