Naslov
Production of lactic acid by lactic acid bacteria Lactobacillus amylovorus DSM 20531T and Lactobacillus coryniformis subsp. torquens DSM 20004T

Autori
Trontel, Antonija ; Jelovac, Nuša ; Pupovac, Marina ; Slavica, Anita ; Novak, Srđan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th Ružička Days "Today Science - Tomorrow Industry" / Šubarić, D. - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2010, 97-97

ISBN
978-953-6894-40-6

Skup
13th Ružička Days "Today Science - Tomorrow Industry"

Mjesto i datum
Vukovar, Hrvatska, 16.09.2010. - 17.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lactic acid; Lactobacillus amylovorus; Lactobacillus coryniformis subsp. torquens

Sažetak
A variety of simpler and complex carbohydrates present in renewable and waste materials may be utilized in the production of lactic acid. The use of pure cultures of lactic acid bacteria capable of fermenting mono- and disaccharides and directly converting polysaccharides to lactic acid without the need for saccharification (hydrolysis of polysaccharides) is advantageous because of the cost and efficiency of the bioprocess. Lactic acid bacteria Lactobacillus amylovorus DSM 20531T and Lactobacillus coryniformis subsp. torquens DSM 20004T were used in this work to produce lactic acid from monosaccharide (glucose), disaccharides (sucrose, maltose or cellobiose) and polysaccharides (starch or cellulose) as a sole carbon and energy sources in the MRS medium. Likewise, combinations of the two carbohydrates (glucose/maltose, glucose/cellobiose, glucose/cellulose, and cellobiose/cellulose) were used for the fermentative production of lactic acid in the medium. Except cellulose, all above mentioned carbohydrates added to the MRS medium (S0 = 10 or 20 g L-1) have been used as substrates by amylolytic lactic acid bacterium L. amylovorus DSM 20531T. High product yields (YP/S = 0.62 – 1.00 g g-1) were reached during overnight incubation at 40C in the medium with glucose, cellobiose and glucose/maltose without pH maintenance. Under the same conditions lactic acid has been produced also by L. coryniformis subsp. torquens DSM 20004T from the substrates and their combinations. More efficient were fermentations of glucose, maltose, sucrose, glucose/maltose, glucose/cellobiose and glucose/cellulose to lactic acid (YP/S = 0.40 - 0.70 g g-1) while significantly lower product yields (YP/S = 0, 03 - 0, 06 g g-1) were obtained in the MRS media with cellobiose, starch, cellulose and cellobiose/cellulose. The production of lactic acid in the MRS medium with cellobiose (S0 = 10 g L-1) by the two bacterial strains was investigated in shaken-flasks experiments at 40C. L. amylovorus DSM 20531T biomass production occurred at maximum specific growth rate (max) of 0, 12 h-1 and, interestingly, cellobiose was almost stoichiometrically (YP1P2/S = 0, 90 g g-1) fermented to lactic acid (P1) and acetic acid (P2).

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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