Naslov
Antifungal effect of tea tree, mountain cedar and wild thyme essential oils to selected Aspergillus species

Autori
Sokolić-Mihalak, Darja ; Kovač, Maja ; Kocevski, Dragana ; Kristek, Suzana ; Gradvol, Vedran ; Pavlović, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Power of Microbes in Industry and Environment 2010 / Hajsig, Danko - Zagreb : Hrvatsko mikrobiološko društvo, 2010, 124-124

ISBN
978-953-7778-00-2

Skup
Power of Microbes in Industry and Environment 2010

Mjesto i datum
Malinska, Hrvatska, 22.09.2010. - 25.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
thyme oil ; tea tree oil ; cedar oil ; antifungal ; Aspergillus

Sažetak
Essential oils are aromatic oily liquids obtained from different plant materials. Some essential oils exhibit antibacterial, antifungal, antiviral, antitoxigenic, antiparasitic and insecticidal properties. Antifungal activity of tea tree, mountain cedar and wild thyme essential oils to three important mycotoxicogenic species of Aspergillus genera (A. carbonarius, A. flavus and A. parasiticus) was evaluated. Minimal inhibitory (MIC) and minimal fungicidal (MFC) concentrations of essential oils were determined in Yeast Extract Sucrose (YES) broth by macro broth dilution method and semisolid YES agar. Results of MIC and MFC obtained by semisolid agar were considerably lower compared to broth method. Wild thyme essential oil inhibited tested fungi at 2500 ppm in semisolid agar while 10 000 to 20 000 ppm was required inhibitory concentration in YES broth. Mountain cedar had no fungicididal effect to analyzed fungal species. Fungi were cultured on Potato Dextrose agar with reduced water activity (aw) levels (0, 95 and 0, 98) and addition of essential oils whereas colony growth inhibition was measured during incubation at 25±0, 5°C. Wild thyme essential oil at 500 ppm prolonged lag phase of A. parasiticus growth to 18 days at 0, 98 aw level, while at 0, 95 aw level lag phase was 10 days. Colony growth of A. carbonarius was reduced at both aw levels by 250 and 500 ppm of thyme oil. Tea tree oil prolonged the fastest growing A. carbonarius to 15 days (4000 ppm, aw 0, 95). Mountain cedar essential oil inhibited linear colony growth of all fungal species, especially A. carbonarius with 7 × slower growth rate while A. flavus and A. parasiticus growth was inhibited similarly (2 – 3 × slower growth rate). Results obtained in the present study indicate the possibility of exploiting essential oils of wild thyme in the fight against important Aspergillus strains, responsible for biodeterioration of stored foods products.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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